Signalling
Part:BBa_K3425107:Design
Designed by: Rubén Crespo Blanco Group: iGEM20_UofUppsala (2020-10-08)
pCadBA testing construct (pSB1C3)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 2049
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2049
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2055 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2049
Illegal XhoI site found at 1033
Illegal XhoI site found at 1925 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2049
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2049
Illegal XbaI site found at 2064
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 2787
Illegal AgeI site found at 2899 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Copy number seems to affect greatly the level of expression attainable by this part. Also, it's important to not have excess expression of BBa_K3425100 to prevent leaky expression. Also, remember that BBa_K3425101 alone does not have an RBS!
Source
Same as BBa_K3425101.