Signalling

Part:BBa_K3425107:Design

Designed by: Rubén Crespo Blanco   Group: iGEM20_UofUppsala   (2020-10-08)


pCadBA testing construct (pSB1C3)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2049
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2049
    Illegal XbaI site found at 2064
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 2787
    Illegal AgeI site found at 2899
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Copy number seems to affect greatly the level of expression attainable by this part. Also, it's important to not have excess expression of BBa_K3425100 to prevent leaky expression. Also, remember that BBa_K3425101 alone does not have an RBS!


Source

Same as BBa_K3425101.

References